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KMID : 0368420010440020087
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Journal of Plant Biology
2001 Volume.44 No. 2 p.87 ~ p.91
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CO_2-Enrichment and Photosynthetic Photon flux Affect the Growth of in Vitro-Cultured Apple Plantlets
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Ru, Yu Li
Murthy, Hosakatte Niranjana/Kim, Seon Kyu/Paek, kee Yeoup
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Abstract
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Micro-cuttings (shoots with two small leaves) of cultivar M9 apple were cultured in-vitro for 40 d under CO_2-enriched and non-enriched (i.e., ambient air) conditions, and at a PPF of 40 or 100μmol m^-2 s^-1. Afterward, shoot length, number of leaves, leaf area, chlorophyll content, shoot and root fresh weights, and % survival were recorded. Those plant-lets grown under CO_2 -and PPF- enriched treatments were healthy and vigorous, and showed higher values for their growth parameters. In contrast, those grown without supplemental CO_2 or PPF often showed hyperhydricity. We also demonstrated that CO_2 enrichment and a relatively high PPF during in-vitro culture promoted normal photosynthesis and growth after ex-vitro transplantation.
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